Inter-Specific Variation Studies among Nephrolepis using SDS-PAGE
نویسندگان
چکیده
منابع مشابه
Tricine – SDS - PAGE Hermann Schägger
Tricine–SDS-PAGE is commonly used to separate proteins in the mass range 1–100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in other electrophoretic systems. These lower concentrations facilitate electroblotting, which is particularly crucial for hydrophobic proteins. Tricine–...
متن کاملMonitoring Cellulase Protein Adsorption and Recovery Using SDS-PAGE
Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was employed to study the sorption behaviors of cellulases on microcrystalline celluloses and hardwood pulp. The adsorption and recovery of cellulases from Aspergillus niger and Trichoderma reesei were investigated at 25 °C. Cellulase recovery was conducted by rinsing adsorbed enzymes with sodium acetate buffer, Milli-Q water,...
متن کاملAnalysis of Genetic Variation among Accessions of Wild and Cultivated Species of Amaranthus Based on Rapd and Sds-page Markers
Genetic diversity and relationships among 12 cultivated and wild Amaranthus species were examined using protein and RAPD markers. High level of genetic diversity was common within species contrary to genetic uniformity within most accessions. On average, the polymorphism reached 42.60% among the cultivars but raised to 46.88% in the wild counterpart. The seed protein content varied in the 11.80...
متن کاملProteomic study of muscle sarcoplasmic proteins using AUT-PAGE/SDS-PAGE as two-dimensional gel electrophoresis.
In the present study, an alternative procedure for two-dimensional (2D) electrophoretic analysis in proteomic investigation of the most represented basic muscle water-soluble proteins is suggested. Our method consists of Acetic acid-Urea-Triton polyacrylamide gel (AUT-PAGE) analysis in the first dimension and standard sodium dodecyl sulphate polyacrylamide gel (SDS-PAGE) in the second dimension...
متن کاملMultiple SDS-PAGE on Vertical Electrophoresis Units.
INTRODUCTION SDS-polyacrylamide gel electrophoresis (SDS-PAGE) represents the second-dimension separation of two-dimensional (2D)-PAGE. Large-scale proteome analysis usually requires simultaneous electrophoresis of batches of second-dimension SDS-PAGE gels to maximize the reproducibility of 2D electrophoresis protein profiles. This requirement is most easily met using multiple, vertical second-...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Pharmacognosy & Natural Products
سال: 2016
ISSN: 2472-0992
DOI: 10.4172/2472-0992.1000112